Molecular Bio 3 – PCR Amplification : Segment of Bacteriophage Lambda DNA
(Molecular Biology 1 and 2 recommended)
In 1983, a biochemist at a small biotechnology company near San Francisco hit upon an idea for replicating DNA in a test tube. It didn’t require an elaborate mix of enzymes. It didn’t require fancy or expensive equipment. It could amplify just a small amount of DNA to over a million fold. It could be used to solve a vast number of biological problems from narrowing down the location of a particular gene within the 3 billion base pairs of the human genome to determining who left a spot of blood on a sidewalk at the scene of a murder. It was so simple, so elegant, so obvious that half the molecular biologists at the time could be heard cursing to themselves, “Now why didn’t I think of that!” Its inventor, Kary Mullis, won a Nobel Prize. The technique is called PCR (for the polymerase chain reaction).
In this camp, a DNA segment of a bacteriophage called lambda will be amplified by PCR. A bacteriophage is a virus that infects bacteria. Lambda (λ), one of the most extensively studied bacteriophages, infects the common intestinal bacterium Escherichia coli. The lambda genome is 48,502 base pairs (bp) in length. You will amplify a 500 bp section of the virus’ genome. PCR is such a powerful technique because of its capacity to amplify very small amounts of DNA.
Ưu đãi
Một số hỗ trợ tài chính có sẵn, xin vui lòng liên hệ để có sẵn.