Molecular Biology 2 – *Protein Purification*
(Molecular Biology 1 recommended)
Molecular Biology is literally illuminated for students in this seminar. Students begin this companion seminar with bacteria that has been genetically transformed using the plasmid, pGLO. Transformed bacteria which produce the genetically engineered Green Fluorescent Protein (GFP) are removed from their agar plates and allowed to multiply in liquid nutrient media. The bacterial cells are then broken open (lysed) to release the Green Fluorescent Protein. GFP is subsequently purified from the contaminating bacterial debris using the disposable chromatography columns. The unique fluorescent properties of GFP allow the entire process to be observed using a long wavelength UV. Students will learn important molecular biology techniques, including: Lab safety, chromatography, and protein isolation and analysis.
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